1. Introduction to Cells & Microscopy
All living things are made of cells, the basic building blocks of life. Because most cells are microscopic, we use microscopes to see them. Light microscopes, which are common in schools, use light and lenses to magnify an object. To work out the size of what you are seeing, you need to understand magnification and be able to use the magnification formula. It is also crucial to be able to convert between different units of measurement, particularly millimetres (mm) and micrometres (µm).
Magnification = Image size / Actual size
Actual size = Image size / Magnification
Image size = Actual size x Magnification
Key term
Examiner insight
Common pitfall
Fun fact
Worked example 13 marks
A student views a palisade cell using a microscope. The image of the cell is 60 mm long. The magnification is x500. Calculate the actual length of the palisade cell in micrometres (µm).
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Step 1: State the formula to be used: Actual size = Image size / Magnification.
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Step 2: Convert the image size units to be the same as the desired answer units. 1 mm = 1000 µm, so 60 mm = 60 x 1000 = 60,000 µm.
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Step 3: Substitute the values into the formula: Actual size = 60,000 µm / 500.
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Step 4: Calculate the final answer: Actual size = 120 µm.
Recap
- Cells are the fundamental units of all living organisms.
- Microscopes are essential tools used to observe cells.
- The magnification formula relates image size, actual size, and magnification.
- Remember the key unit conversion: 1 millimetre (mm) = 1000 micrometres (µm).
- Always show your working in calculation questions.
Quick check
- An image is 100 times larger than the object. What is the magnification?1 mark
- Convert 0.25 mm into µm.1 mark